Test Preparation on Biotechnology - Biology Test Series for NEET - 2020

1.

The linking of antibiotic resistance gene with the plasmid
vector became possible with

1DNA polymerase
2Exonucleases
3DNA ligase
4Endonucleases

2.

Main objective of production./use of herbicide resistant GM
crops is to

1Encourage eco-friendly herbicides
2Reduce herbicide accumulation in food articles for health safety
3Eliminate weeds from the field without the use of manual labour
4Eliminate weeds from the field without the use of herbicides

3.

Which of the following is commonly used as a vector for
introducing a DNA fragment in human lymphocytes

1Arbovirus
2Rotavirus
3Enterovirus
4Retrovirus

4.

Branch dealing with genetic engineering is

1Eugenics
2Euthenics
3Euphenics
4None of these

5.

Who among the following scientists is associated with the
discoveries in genetic engineering

1Khorana
2Watson
3Crick
4Messelson

(a) Hargovind Khorana is associated with genetic
engineering. He synthesized ‘gene’ artificially’ in a test
tube (1969).

6.

Which of the following organelles is related with genetic
engineering/gene cloning

1Golgi apparatus
2Lysosomes
3Mitochondria
4Plasmids

(d) Plasmids are extrachromosomal covalently closed
circular double stranded molecules of DNA present in
most prokaryotes. Therefore they are used as a vector
in genetic engineering.

7.

In genetic engineering, a DNA segment (gene) of interest, is
transferred to the host cell through a vector. Consider the
following four agents (A-D) in this regard and select the
correct option about which one or more of these can be
used as a vector/vectors
Statements
(A) A bacterium (B) Plasmid
(C) Plasmodium (D) Bacteriophage
Options

1(A), (B) and (D) only
2(A) only
3(A) and (C) only
4(B) and (D) only

(d) Plasmids and bacteriophages are used as vectors in
genetic engineering.

8.

Recombinant DNA (rDNA) technology is related with

1C. Darwin
2Stanley Cohen
3Herbert Boyer
4Both (b) and (c)

(d) The first recombinant DNA was constructed by
Stanley cohen and Herbert in 1972. They cut the
piece of DNA from a plasmid carrying antibioticresistance
gene in the bacterium Salmonella
typhimurium and linked it to the plasmid of
Escherichia coli.

9.

Identify the plasmid

1AIU I
2Hind III
3Eco RI
4pBr 322

(d) Plasmid pBr 322 was one of the first widely used
cloning vectors, it contains both ampicillin and
tetracycline resistance genes.


Right Ans	Wrong Ans	Not Attempted

Test Preparation on Biotechnology - Biology Test Series for NEET - 2020

The linking of antibiotic resistance gene with the plasmidvector became possible with

Main objective of production./use of herbicide resistant GMcrops is to

Which of the following is commonly used as a vector forintroducing a DNA fragment in human lymphocytes